To investigate the immunomodulatory mechanism of action of BPIFA1 and BPIFB1, we performed whole transcriptome sequencing of airway epithelial cells pretreated with recombinant BPIFA1 or BPIFB1 with or without stimulation with P. aeruginosa. Although the cells were responsive to stimulation with P. aeruginosa, we found that pretreatment with BPIFA1 and BPIFB1 had little effect on the response to infection. Here, BPIFA1 is linked to infection.