As shown in Figure 3A,3, Western blotting analysis revealed that HSF1, a key regulator of Hsps expression, was significantly increased in ET group and obviously down‐regulated in TAC group relative to Sham group, the exercise training displayed a marked rise in the protein level of HSF1 in TAC + ET mice compared with TAC mice. The gene discussed is HSF1; the disease is persistent truncus arteriosus.