As changes in the circulation may not accurately reflect neutrophil infiltration into the brain, absolute neutrophil counts in the ischemic brain 24 hours after stroke onset were quantified by flow cytometry (Figure 2E, 2F), with neutrophils identified as CD45High/CD11b+/Ly6G+ cells (gating strategy shown in Supplementary Figure 2). Here, ITGAM is linked to stroke disorder.