As regard to the association between gene promoter region methylation and different cancer stages for 5-year OS of patients with CRC, no significant relationship was observed between CDKN2A, hMLH1, and CSF2 methylation in the advanced cancer stages (Me/advanced) and cancer mortality in 5 years in both normal and tumor tissue (data not shown). The gene discussed is MLH1; the disease is colorectal carcinoma.