CALB1 and Anxiety: To explore the role of Calb1 in the anxiolytic effect of the pBLA–vCA1 circuit, we microinjected adeno-associated virus (AAV)-shCalb1 or the control AAV-shNT (encoding a nontargeting shRNA) into the vCA1 of wild-type mice and measured anxiety behaviours (Supplementary Fig. 16a–c).