Our real-time PCR also revealed that the PD-L1 expression was significantly decreased in Lgr5/Arf1/Apc mice (Supplementary Fig. 4w), in the liver of MYO-ON mice treated with GCA compared with DMSO (Supplementary Fig. 4x), and in the tumor of BALB/c mice injected with murine colon carcinoma CT26 cells treated with GCA (Supplementary Fig. 4y) or with murine breast carcinoma 4T1 cells treated with GCA (Supplementary Fig. 4z). Here, LGR5 is linked to colon carcinoma.