Under these treatments, the tumor numbers significantly increased (Lgr5/Apc: 101.2 ± 9.6; Lgr5/Arf1/Apc: 53.1 ± 7.7; ARL/Lgr5/Arf1/Apc: 50.2 ± 10.6; TLR4i/Lgr5/Arf1/Apc: 69.2 ± 11.7; OxATP/Lgr5/Arf1/Apc: 80.6 ± 10.5; OxATP/Lgr5/Arf1/Apc: 82.6 ± 7.0), and the mouse lifespan decreased, while the overall body weight did not change significantly, compared with mice without inhibitor treatment (Fig. 6b, c, and Supplementary Fig. 9a). This evidence concerns the gene ARF1 and neoplasm.