HMGB1 and neoplasm: To examine whether DAMPs are essential for the anti-tumor immune response of Arf1 ablation, we first interfered with the action of ATP and HMGB1 in Lgr5/Arf1/Apc intestinal CSC mice by administering ARL61756 (ARL), oxATP, suramin, and the Toll-like receptor 4 (TLR4) inhibitor (Fig. 6a, b).