Based upon our findings from gene expression profiling and since previous studies showed that the hydrocephalus phenotype in Cdkn1c cKO induced with Nestin-Cre can be rescued by concomitant p53 (Trp53) ablation15, we next analyzed cell death parameters upon conditional loss of Cdkn1c. We thus stained cortex at E13 in MADM-7, Cdkn1c-MADM-7 and cKO-Cdkn1c-MADM-7 embryos for apoptotic cells with antibodies against Caspase-3 (Fig. 5). Here, TP53 is linked to Hydrocephalus.