Next, we performed loss- and gain-of-function assays, and detected that the knockdown or overexpression (Fig. 2d, e), decreased or increased TEAD4 enrichment on the MNX1-AS1 promoter respectively (Fig. 2i, h), contributing to MNX1-AS1 downregulation or upregulation in GC cells, respectively (Fig. 2f). Here, TEAD4 is linked to gastric cancer.