Based on our findings that hydrogen treatment increased the expression of pyroptosis-related protein NLRP3, ASC, pro-caspase-1, and caspase-1 in endometrial cancer cells (Fig. 3), and that hydrogen-treated cells exhibited higher populations of PI- and TUNEL-positive cells, we propose that pyroptosis by DNA fragmentation is mediated by ROS and the NLRP3 inflammasome (Figs. 4, 5 and 6). This evidence concerns the gene NLRP3 and endometrial cancer.