Hydrogenated culture medium (H-CM) was added to Ishikawa, HEC1A, and AN3CA endometrial cancer cell lines, and we identified significant statistical difference in NLRP3, ASC, caspase-1 and GSDMDNterm on relative density histogram between H-CM and normal culture medium (CM) group of ishikawa and HEC1A cells as detected by representative western blotting analyses (P < 0.05) (Fig. 3a). This evidence concerns the gene CASP1 and endometrial cancer.