In this research, the size and shape of plasma EVs were analysed via TEM, and TSG101 and CD63 (exosome markers) were used to verify exosomes.25 After the measurement of the expression level of circ‐0000190 in plasma EVs, it was interestingly found that the level of EVs circ‐0000190 was decreased in osteosarcoma patients. This evidence concerns the gene TSG101 and osteosarcoma.