Single round infection in SupT1 WT or SupT1 EAP45 KO cells using HIV GFP‐expressing virus pseudotyped with VSV‐G shows a modest defect in viral gene expression possibly at a post‐transcriptional level (Figure 2c) consistent with our published results in HAP1 EAP45 KO cells (Meng et al., 2015). Here, HAP1 is linked to infection.