To determine whether GPC3-Syn-IL12-NK92 cells could affect lysis of GPC3-positive HCC cells by GPC3-28Z CAR T cells in vitro, cytotoxicity assays were performed by incubating GPC3-28Z CAR-T cells with each of the four HCC cell lines and keeping the ratio of NK92 cells and T cells at 1:1. This evidence concerns the gene GPC3 and hepatocellular carcinoma.