To confirm whether or not Rap1b functions as an oncogene in glioma, we assessed the effects of Rap1b on glioma cell proliferation and invasion in vitro using MTT assay or Matrigel transwell invasion assay after transfecting A172, U87MG, U373MG, and SNB19 with synthesized specific small interfering RNAs (siRap1b) targeting Rap1b mRNA. This evidence concerns the gene RAP1B and glioma.