An annexin apoptosis assay showed that RIZ1 significantly increased the number of Annexin V positive cells in HeLa-RIZ1-AE and SiHa-RIZ1-AE compared with HeLa-vector and SiHa-vector (both P < 0.001, Figures 6C,D), suggesting that overexpression of RIZ1 induces apoptosis in cervical cancer cells. This evidence concerns the gene ANXA5 and cervical cancer.