Using mouse embryonic fibroblasts (MEFs) and human malignant melanoma cells treated with active-site mTOR inhibitor (asTORi), was demonstrate that mTORC1 stimulates the translation of MTFP1 mediated by 4E-BP, and therefore the mTOR inhibition induces the phosphorylation of the DRP1 at Ser 637, this phosphorylation prevents it translocation to mitochondria, conversely, the pro-fission phosphorylation site of DRP1 at Ser 616 was decreased in asTORi treated cells. This evidence concerns the gene MTOR and melanoma.