TNF-α secretion from cancer cell line increased expression of MMP-2 and MMP-9 and increased TNF-α production. A TNF-α/TNF-R1/NF-kB system signaling pathway generated a highly metastatic cancer cells. TNF-α-triggered NF-κB activation to upregulation of active MMP released from the cancer cells. This evidence concerns the gene MMP2 and cancer.