To detect the effects of SMAD5-AS1, miR-195, and SMAD5 on NPC cell proliferation and apoptosis, EdU assay and flow cytometry were adopted to detect the proliferation and apoptosis of CNE-2 and CNE-1 cells, respectively, and western blot analysis was used to detect the protein expression of apoptosis-related factors (Bax and Bcl-2) in CNE-2 and CNE-1 cells. The gene discussed is BAX; the disease is nasopharyngeal carcinoma.