Next, CNE-2 and CNE-1 cells were infected with lentivirus expressing oe-BMP2 to overexpress BMP2 and RT-qPCR and Western blot analysis were used to determine the expression of SMAD5-AS1 and miR-195 and mRNA and protein expression of BMP2 and SMAD5 following infection. This evidence concerns the gene SMAD5 and infection.