To figure out the effect of SMAD5-AS1, miR-195 and SMAD5 on migration, invasion and EMT in NPC cells, Transwell assay was applied to detect CNE-2 and CNE-1 cell migration and invasion abilities, and western blot analysis was adopted to detect the expression of EMT markers Vimentin and E-cadherin in CNE-2 and CNE-1 cells. The gene discussed is SMAD5; the disease is nasopharyngeal carcinoma.