CACNA1D and neurodevelopmental disorder: Although our data strongly support CACNA1D as a high-risk gene for neurodevelopmental disorders and emphasize the need of functional analysis to distinguish likely pathogenic (able to increase Cav1.3 activity) from non-pathogenic de novo mutations (unable to increase Cav1.3 activity), our studies do not provide insight into altered signaling cascades downstream of Cav1.3 channels.