In accord with NanostringTM data, qPCR analysis in non-injected tumors treated with the combination demonstrated significant up-regulation of Th1 cytokines and chemokines (Tnf, Il1b, Il6, Il18, Ifng, Ccl5, Cxcl9 and Cxcl10), CD8 (Cd8b), a key costimulatory molecule (Cd80) and a cell adhesion molecule known to be important for T cell recruitment to the tumor bed (Icam1). This evidence concerns the gene IL18 and neoplasm.