Using the xCELLigence impedance-based system we found that both CD8-GFP and CD8-EGFR-GFP T cells were able to recognize and kill B16-OVA cells in a similar way when the effector to target ratio was relatively high (0.5 effector T cells per 1 tumor cell) (Figure 3D, first graph). This evidence concerns the gene CD8A and neoplasm.