Flow cytometric analysis showed that the frequency of CD4+RORγt+ cells (Th17) was significantly decreased in pectin-fed mice compared with control mice; however, there was no significant difference between citrus and orange pectin (Figure 2A), suggesting that the protective effect of orange pectin against TNBS colitis was not attributable to suppression of Th17 differentiation. The gene discussed is CD4; the disease is colitis.