We also found that Tregs in CRC TME comprise mainly of FoxP3+Helios+ Tregs, which were significantly higher in the TME compared with normal tissue and periphery (5.7 ± 0.6 vs. 6.6 ± 0.5 vs. 20.5 ± 2.3, Figure 1E). This evidence concerns the gene FOXP3 and colorectal carcinoma.