CD4 and neoplasm: Moreover, whereas Ly6G stainings were located within the primary tumor area at all times, CD11c stainings for TADCs were initially located in the tumor stroma at 1 w p.i. and subsequently invaded the primary tumor core by 3 and 6 w p.i. Regarding the adaptive immune cells, immunohistochemistry showed significant increase in CD3ε stainings for tumor-infiltrating lymphocytes (TILs), in CD8a stainings for cytotoxic T-cells, and in CD4 stainings for T-helper cells in the primary tumor area of the 4T1- compared to Py230-based model at 1 and 3 w p.i., but not at 6 w p.i. (Figure 7A).