It is worth to note that we have also verified the effect of the PI3K/Akt/FOXO3 axis on the expression of TNF-α, a cytokine that plays a crucial role in TB immune response, and found that neither the used inhibitors nor the FOXO3 silencing had an effect on BCG-induced secretion of TNF-α (Figure S3). Here, AKT1 is linked to tuberculosis.