Further comparisons of the AMs derived from different patient groups, we found that the macrophage signal could be stratified according to health status where COPD- and smoker-derived AMs displayed a higher M-IL4, M-IL10, and M-dex signal and a reduced M-IFNγ and M-LPS+IFNγ signal compared to AMs obtained from HCs. Here, IFNG is linked to chronic obstructive pulmonary disease.