Similarly, in CRISPR screens with two different murine cancer cell lines, MC38 colon adenocarcinoma and B16 melanoma cells, which had been engineered to present chicken ovalbumin (Ova) antigen, IFN-γ signaling and antigen presentation were also found to be critical for cytotoxic T cell-mediated killing (23). The gene discussed is IFNG; the disease is melanoma.