ITGAE and neoplasm: In addition, coculture with primary NSCLC cells downregulated the differentiation of the CD1c+CD205+CD103+ DC subset compared with that of DCs without incubation with primary tumor cells (Figure 3C); however, incubation with primary tumor cells facilitates the development of the CD1c+CD205+CD103− DC subpopulation compared with that without coculture with primary NSCLC cells (Figure 3D).