IFNG and neoplasm: Both TIL recognized autologous tumor cells and responded with degranulation and production of IFN-γ, which was measured by FACS as increased cell surface CD107a and intracellular IFN-γ (Supplementary Figure 1A) or by ELISA as secreted IFN-γ in supernatants [Figure 1A (KADA) and Figure 1D (ANRU)].