From these metabolic pathways, we concluded that the HF diet decreases Map2k4 DNA methylation and increases Irs2 DNA methylation, as all of the HF-CON, CON-HF, and HF-HF groups generally had lower Map2k4 DNA methylation and higher Irs2 DNA methylation than the CON-CON group. The gene discussed is MAP2K4; the disease is hydrops fetalis.