As shown in Figure 6C, the level of NES1 in the PVN of the hypothalami from rats in the CIS group was markedly higher than that in the control group (P < 0.01), whereas the levels of NES1 in the Xiaoyaosan group and fluoxetine group were significantly lower than those in the CIS group (P < 0.01 and P < 0.05, respectively). This evidence concerns the gene KLK10 and in situ carcinoma.