As shown in Figure 4B, the NES1 level in the CIS group was significantly higher than that in the control group (P < 0.01), and both Xiaoyaosan and fluoxetine significantly inhibited this increase in NES1 (P < 0.01 and P < 0.01, respectively) compared with CIS alone. This evidence concerns the gene KLK10 and in situ carcinoma.