The hepatoma cell line cell line HepG2, which is widely used not only for liver-associated research, seeded on polystyrene well plates with and without coating exhibited significant differences in their morphology, distribution, and functions such as particle uptake or P450-dependent detoxification (Saravia and Toca-Herrera, 2009; Prats-Mateu et al., 2014). This evidence concerns the gene CYP2B6 and hepatocellular carcinoma.