Thus, our major purpose of this study was to test the involvement of Kv7.4 in auto-inhibition and compare its contribution to that of Kir3.2, in the VTA DA neurons projecting to NAc, because of the prominent role of this projection in the development of depression behavior (Cao et al., 2010; Chaudhury et al., 2013). This evidence concerns the gene KCNQ4 and major depressive disorder.