Next, we used in vitro oxygen glucose deprivation (OGD) models in HT-22 cells and BV2 cells to confirm those above effects of meisoindigo and the underlying TLR/NF-κB signaling pathway against cerebral ischemia reperfusion injury (CIRI) by co-treatment with a combination of meisoindigo and LPS Our results showed that meisoindigo may protect against cerebral ischemic injury in the brain by suppressing NLRP3 inflammasome activation and M1 polarization via inhibiting TLR/NF-κB signaling pathway, which is expected to be a promising new drug candidate for the treatment of ischemic stroke. This evidence concerns the gene NFKB1 and ischemic stroke.