To explore the molecular signaling mechanism by which meisoindigo inhibits NLRP3 inflammasome activation and blocks M1 microglia polarization, we measured the expression of TLR-4/NF-κB signaling pathway, a key intracellular signaling system that plays an important role in the initiation of the immune response in the ischemic stroke brain after meisoindigo treatment. This evidence concerns the gene TLR4 and ischemic stroke.