As exhibited in Fig. 1d, BCYRN1 was remarkably up-regulated in HCC cell lines in contrast with the control, and the expression was highest in HepG2 cells and lowest in BEL-7402 cells, so HepG2 and BEL-7402 were picked up for following experiments (P < 0.01). This evidence concerns the gene BCYRN1 and hepatocellular carcinoma.