FGD5 and non-small cell lung carcinoma: And then, to investigate the precise function of FGD5-AS1 in NSCLC, we carried out loss-of-function assays after confirming the inhibition of FGD5-AS1 in NCI-1703 and NCI-1793 cells by qRT-PCR analysis in Figure 1D. Also, CCK-8 experiment and clone formation assay were performed to assess the proliferation of NSCLC cell according to different group of interfered FGD5-AS1 (Figure 1E,F).