As the symptoms (e.g., muscle atrophy, paralysis and NMJ dysfunction) of the Nampt−/− cKO mice resemble those in ALS model and NAMPT is downregulated in human ALS brain22, the current study provides novel insights for the involvement of the NAMPT-mediated NAD+ salvage pathway in the pathology of ALS. This evidence concerns the gene NAMPT and amyotrophic lateral sclerosis.