To test if the four genes are required for efficient virus production, we infected WDR7, CCDC115, TMEM199, and CMTR1 polyclonal KO cells with H1N1 PR8 virus and H3N2 Udorn virus at MOI 0.1 and monitored virus production at 24, 48, and 72 h post-infection by plaque assay. Here, VMA22 is linked to infection.