To determine if expression levels of genes regulated by DEAF1 were also reduced in GVHD, we used gene set enrichment analysis (GSEA) to determine enrichment or otherwise of 157 DEAF1-dependent genes (defined as genes with ≥ 3-fold reduced expression in LN stromal cells from Deaf1-knockout versus wild-type mice) (18). This evidence concerns the gene DEAF1 and graft versus host disease.