To demonstrate that the CD33+ HLA-DR-/low MDSCs are an immunoregulatory subset of cells, we did an intracellular staining of IL-10 and TGF-β and we found that this subpopulation actually produces these cytokines (Figure 2), suggesting that the immunosuppressive function of the MDSCs is also increased in the DM2 patients. The gene discussed is TGFB1; the disease is myotonic dystrophy type 2.