AFP and hepatocellular carcinoma: Similarly, the frequency of the rs1169310-TT+CT genotypes was significantly greater in the AFP ≤ 400 ng/ml group than in the AFP > 400 ng/ml group (84.7% vs. 67.2%, P = 0.005), and the rs1169310-CC frequency was significantly lower (15.3% vs. 32.8%, P = 0.005), suggesting that HCC patients carrying the rs1169310-T mutation allele also were more likely to have a serum AFP concentration ≤ 400 ng/ml.