In T3 vs. T1 group, the most downregulated protein was complement C4-A (C4A) in the pathways of Complement and coagulation cascades, Staphylococcus aureus infection, and Systemic lupus erythematosus with thrombospondin-1 (THBS1) in Phagosome and Focal adhesion pathways, tropomyosin alpha-4 (TPM4) in Dilated cardiomyopathy and Hypertrophic cardiomyopathy pathways, vinculin (VCL) in Amoebiasis and Regulation of actin cytoskeleton pathways, and immunoglobulin heavy variable 6-1 (IGHV6-1) in Hematopoietic cell lineage. The gene discussed is THBS1; the disease is hypertrophic cardiomyopathy.