Although the mRNA expression of both RIPK3 and MLKL was upregulated in CCA tissues (n = 36) compared to the level in normal bile ducts (n = 9), there was no correlation between RIPK3 or MLKL expression and overall survival (OS) or disease-free survival (DFS) as analyzed by the GEPIA online tool (S9 Fig); this discrepancy is partly explained by the limited number of CCA patients in TCGA database. This evidence concerns the gene MLKL and cholangiocarcinoma.