To extend these observations beyond the HEK TER cells, we generated a MAP4K4 knockdown gene expression signature and assessed this signature across a large collection of cancer cell lines from the Cancer Cell Line Encyclopedia (CCLE) by performing ssGSEA analysis (Figure 8C–D) (Barbie et al., 2009; Barretina et al., 2012). This evidence concerns the gene MAP4K4 and cancer.