CD4 and melanoma: Flow cytometry analysis performed on tumors collected 11 days after melanoma cell inoculation, a time point when tumors begin to shrink in Siah2−/− mice (Supplementary Fig. 2a) revealed a comparable number (Fig. 2a) or proportion (Fig. 2b) of CD45.2+, CD4+, CD8+, CD11b+ F4/80+, CD11c+, and CD11b+GR1+ cells in both genotypes (Fig. 2a, b, Supplementary Fig. 2b).