In this study, we generated a new tauopathy mouse model by inoculating distinct tauopathy brain-derived pathological tau strains into a new mouse line 6hTau that express equal ratio of 3R and 4R tau isoforms, to study the intrinsic properties of Htau strains that are responsible for distinct tau isoform recruitment, cell-type specificity and transmission in human neurodegenerative tauopathies. This evidence concerns the gene MAPT and tauopathy.