The expression profiles of profibrotic genes such as CYGB, ACTA2, PCDH7, DES, COL1A1, and COL1A3 were also significantly up‐regulated in the co‐culture NASH model, when compared with the steatosis model (Fig. 1G). This evidence concerns the gene COL1A1 and metabolic dysfunction-associated steatohepatitis.