The I148M SNP in the PNPLA3 gene is highly associated with an increased risk of developing hepatic steatosis, NASH, and liver fibrosis.3, 25 Recently, a role for the mutated protein has been revealed in HSCs, where it has been shown to cause these cells to increase their activation status, become more pro‐inflammatory, and have a higher lipid content.35 By taking HSC donors that contained the WT or mutant I148M PNPLA3 allele, we were able to confirm these findings using our in vitro MPS NASH model. This evidence concerns the gene PNPLA3 and fatty liver disease.