PNPLA3 and metabolic dysfunction-associated steatohepatitis: The study by Bruschi et al. was performed only on immortalized hepatic cell lines and on monocultures of isolated primary HSCs.35 Here we have demonstrated using a more complete in vitro recapitulation of the liver, exhibiting a disease phenotype in which the I148M PNPLA3 mutation in HSCs can enhance the overall NASH disease state.