In conclusion, our study illuminated that IRS1/PI3K/AKT pathway signal involved in the regulation of metabolic abnormalities by mulberry leaf extracts flavonoids in 3T3-L1 adipocytes insulin resistance model and suggested that flavonoids extracted from mulberry leaves may be a candidate for the therapeutic option for T2DM. The gene discussed is IRS1; the disease is type 2 diabetes mellitus.