Lung cancer cells (A549 and H1299) showed increased mRNA and protein levels of mesenchymal cell markers, such as TWIST1, Snail, Slug, ZEB1, Bmi1, fibronectin, N‐cadherin, vimentin, and VEGF, after ISX induction using doxycycline (Dox.; 1 μg/ml) but decreased expression of epithelial cell marker E‐cadherin (Fig 1A and B). This evidence concerns the gene SNAI2 and lung cancer.