A partial correlation was calculated to analyze whether TMAO correlated with PCOS without HA by controlling for the confounding factor BMI, and the results showed that the plasma levels of TMAO (r = 0.423, P < 0.01), LH (r = 0.482, P < 0.01), LH/FSH (r = 0.460, P < 0.01), and FBG (r = 0.408, P < 0.01) were positively correlated with the incidence of PCOS without HA. Here, PLOD1 is linked to polycystic ovary syndrome.