To evaluate the expression profile of NEK1, NEK2, NEK3, and NEK5 in different types of cancer an immunohistochemistry assay was conducted in 10 common types of cancer (esophagus, stomach, colon, lung, thyroid, breast, uterine cervix, pancreas, prostate and kidney) with 4 cases of each and their matched normal tissue. The gene discussed is NEK2; the disease is cancer.